Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb【科瑞奥博】

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Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb

货号： 39359S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	39359S
同种亚型	Rabbit IgG
反应种属	H
来源宿主	Rabbit IgG
应用	W IF-IC
目标/特异性	Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb recognizes endogenous levels of keratin 20 protein only when phosphorylated at Ser13.
使用方法	WB(1:1000) IF-IC (1:400)

性能
供应商	CST
灵敏度	Endogenous
背景	Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
运输条件	0.75
存放说明	-20C
计算分子量	48
参考文献	1 . Moll, R. et al. (1982) Cell 31, 11-24. 2 . Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13. 3 . Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4. 4 . Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66. 5 . Zatloukal, K. et al. (2004) J Pathol 204, 367-76. 6 . Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85. 7 . Zhou, Q. et al. (2006) J Biol Chem 281, 16453-61. 8 . Menon, M.B. et al. (2010) J Biol Chem 285, 33242-51. 9 . Moll, R. et al. (1992) Am J Pathol 140, 427-47. 10 . Karantza, V. (2011) Oncogene 30, 127-38.

参考图片

Confocal immunofluorescent analysis of HT-29 cells, untreated (left), treated with Anisomycin #2222 (25 μg/mL, 30 minutes; center), or treated with anisomycin followed by λ phosphatase (right), using Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from of HT-29 cells, untreated (-), treated with Anisomycin #2222 (25 μg/ml, 30 min; +), or treated with Anisomycin and Calf Intestinal Phosphatase (CIP)/λ phosphatase (+), using Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb (upper), Keratin 20 (D9Z1Z) XP^® Rabbit mAb #13063 (middle) or β-Actin (D6A8) Rabbit mAb #8547 (lower).