| 货号 | 34911S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H |
| 来源宿主 | Rabbit IgG |
| 应用 | ChIP |
| 目标/特异性 | Phospho-Stat3 (Ser727) (D4X3C) Rabbit mAb is recognizes endogenous levels of Stat3 protein only when phosphorylated at Ser727. |
| 使用方法 | ChIP(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 86 |
| 参考文献 | 1 . Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. 2 . Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. 3 . Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. 4 . Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. 5 . Bromberg, J.F. et al. (1999) Cell 98, 295-303. 6 . Darnell, J.E. et al. (1994) Science 264, 1415-21. 7 . Ihle, J.N. (1995) Nature 377, 591-4. 8 . Wen, Z. et al. (1995) Cell 82, 241-50. 9 . Yokogami, K. et al. (2000) Curr Biol 10, 47-50. 10 . Biethahn, S. et al. (1999) Exp Hematol 27, 885-94. |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with IL-6 (100ng/mL) for 30 minutes and either 10 μl of Phospho-Stat3 (Ser727) (D4X3C) Rabbit mAb (ChIP Specific) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
