| 货号 | 24129S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | M |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit mAb (Mouse Specific) recognizes endogenous levels of mouse IRF-7 protein when dually phosphorylated at Ser437 and Ser438. This antibody has a preference for Ser437 and can recognize single phosphorylation at this site. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55. 2 . Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58. 3 . Au, W.C. et al. (1998) J Biol Chem 273, 29210-7. 4 . Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18. 5 . Marié, I. et al. (1998) EMBO J 17, 6660-9. 6 . Sato, M. et al. (2000) Immunity 13, 539-48. 7 . Honda, K. et al. (2005) Nature 434, 772-7. 8 . Colina, R. et al. (2008) Nature 452, 323-8. 9 . Lin, R. et al. (2000) J Biol Chem 275, 34320-7. 10 . Yang, H. et al. (2003) J Biol Chem 278, 15495-504. 11 . Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7. |
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or transfected with poly(deoxyadenylic-deoxythymidylic) acid (dA:dT, 5 μg/ml, overnight; +), using Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit mAb (Mouse Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing full-length Myc/DDK-tagged mouse IRF-7 protein (mIRF-7-Myc/DDK; +) or GST-tagged human IKK-ε protein (hIKK-ε-GST; +), using Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit mAb (Mouse Specific). A human specific background band is detected at 40 kDa in 293T cells |
