| 货号 | 53044S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse |
| 应用 | WB,IP |
| 目标/特异性 | Phospho-cPLA2 (Ser505) (D4I2A) Rabbit mAb recognizes endogenous levels of cPLA2 protein only when phosphorylated at Ser505. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid (1). cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity (1). Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane (2). Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 (3,4) and Ser727 (5) stimulates its catalytic activity. |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Leslie, C.C. (1997) J. Biol. Chem. 272, 16709-16712. 2 . Clark, J. D. et al. (1991) Cell 65, 1043-1051. 3 . Lin, L. L. et al. (1993) Cell 72, 269-278. 4 . Kramer, R.M. (1996) J. Biol. Chem. 271, 27723-27729. 5 . Hefner, Y. et al. (2000) J. Biol. Chem. 275, 37542-37551. |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with λ-phosphatase (+), using Phospho-cPLA2 (Ser505) (D4I2A) Rabbit mAb (upper) or cPLA2 (D11H4) Rabbit mAb #5479 (lower). | |
Western blot analysis of extracts from NIH/3T3 and C2C12 cells using Phospho-cPLA2 (Ser505) (D4I2A) Rabbit mAb (upper) or cPLA2 (D11H4) Rabbit mAb #5479 (lower). |
