| 货号 | 98195S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP |
| 目标/特异性 | Phospho-MEK1 (Ser298) (D1P9E) Rabbit mAb recognizes endogenous levels of MEK1 protein only when phosphorylated at Ser298. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. MEK1 is phosphorylated at Ser298 by PAK1, which facilitates signal transduction from Raf to MEK1 and Erk2 (5-7). MEK1 is also phosphorylated by cdk5 at Thr286 in mitotic cells, causing negative feedback of the p44/42 MAP kinase pathway (8). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Crews, C.M. et al. (1992) Science 258, 478-480. 2 . Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19. 3 . Rosen, L.B. et al. (1994) Neuron 12, 1207-21. 4 . Cowley, S. et al. (1994) Cell 77, 841-52. 5 . Xu Be et al. (2001) J Biol Chem 276, 26509-15. 6 . Eblen, S.T. et al. (2002) Mol Cell Biol 22, 6023-33. 7 . Sharma, P. et al. (2002) J Biol Chem 277, 528-34. 8 . Coles, L.C. and Shaw, P.E. (2002) Oncogene 21, 2236-44. |
Western blot analysis of extracts from HeLa cells, either wild-type (+/+) or MEK1 knockout(-/-), using Phospho-MEK1 (Ser298) (D1P9E) Rabbit mAb (upper), MEK1 (D2R1O) Rabbit mAb #12671 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from HT-29 and H-4-II-E cells using Phospho-MEK1 (Ser298) (D1P9E) Rabbit mAb. The phospho-specificity of the antibody was verified by pre-incubating the antibody with no peptide (left), MEK1 (Ser298) non-phosphopeptide (center), and MEK1 (Ser298) phosphopeptide (right). | |
Western blot analysis of extracts from MDA-MB-231, NIH/3T3, and H-4-II-E cells, either untreated (-) or treated with phosphatase (+), using Phospho-MEK1 (Ser298) (D1P9E) Rabbit mAb (upper) or MEK1 (D2R1O) Rabbit mAb #12671 (lower). | |
Western blot analysis of extracts from MEF cells, either wild-type (+/+) or Mek1 knock-out (-/-), using Phospho-MEK1 (Ser298) (D1P9E) Rabbit mAb (upper), MEK1 (D2R1O) Rabbit mAb #12671 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Mek1 KO MEFs were generously provided by Dr. Jean Charron, Centre de recherche du Centre hospitalier de lUniversité Laval, Quebec, Canada. |
