| 货号 | 74129S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB |
| 目标/特异性 | Phospho-Jak1(Tyr1034/1035) (D7N4Z) Rabbit mAb recognizes endogenous levels of Jak1 protein only when phosphorylated at Tyr1034/1035. This antibody also detects Jak1 when phosphorylated at just Tyr1034. Human Jak1 residues Tyr1034 and Tyr1035 historically have been referenced as Tyr1022 and Tyr1023. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Leonard, W.J. and OShea, J.J. (1998) Annu Rev Immunol 16, 293-322. 2 . Darnell, J.E. (1997) Science 277, 1630-5. 3 . VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93. 4 . Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92. 5 . Zhu, T. et al. (1998) J Biol Chem 273, 10682-9. |
Western blot analysis of extracts from various cell lines, serum-starved overnight (-) followed by treatment with Human Interferon-α1 #8927 (hIFN-α1, 10 ng/ml, 15 min; +) or Human Interleukin-4 #8919 (hIL-4, 10 ng/ml, 10 min; +) using Phospho-Jak1 (Tyr1034/1035) (D7N4Z) Rabbit mAb (upper) or Jak1 (6G4) Rabbit mAb (lower). |
