| 货号 | 58052S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC |
| 目标/特异性 | Phospho-SMC1 (Ser957) (D7S8Y) Rabbit mAb recognizes endogenous levels of SMC1 protein only when phosphorylated at Ser957. |
| 使用方法 | W IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
| 参考文献 | 1 . Michaelis, C. et al. (1997) Cell 91, 35-45. 2 . Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5. 3 . Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82. 4 . Kim, S.T. et al. (2002) Genes Dev 16, 560-70. |
Confocal immunofluorescent analysis of 293 cells, untreated (left), UV-treated (middle), or UV-treated followed by λ phosphatase (right), using Phospho-SMC1 (Ser957) (D7S8Y) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of extracts from 293 and 3T3 cells, untreated (-) or UV-treated (+), using Phospho-SMC1 (Ser957) (D7S8Y) Rabbit mAb (upper) or SMC1 (8E6) Mouse mAb #6892 (lower). |
