| 货号 | 68790S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb recognizes endogenous levels of Tyk2 protein only when phosphorylated at Tyr1054 and Tyr1055. Cross-reactivity was not observed with other Jak family members. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tyk2 is a member of the Jak family of protein tyrosine kinases. It associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and IFN-α and β (1-3). Following ligand binding, Tyk2 is activated by phosphorylation of Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-β signaling cascade (5). |
| 存放说明 | -20C |
| 计算分子量 | 134 |
| 参考文献 | 1 . Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-34. 2 . Stahl, N. et al. (1994) Science 263, 92-5. 3 . Leonard, W.J. and OShea, J.J. (1998) Annu Rev Immunol 16, 293-322. 4 . Gauzzi, M.C. et al. (1996) J Biol Chem 271, 20494-500. 5 . Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-11. 6 . Strobl, B. et al. (2011) Front Biosci (Landmark Ed) 16, 3214-32. 7 . Karaghiosoff, M. et al. (2000) Immunity 13, 549-60. 8 . Shimoda, K. et al. (2000) Immunity 13, 561-71. 9 . Minegishi, Y. et al. (2006) Immunity 25, 745-55. |
Western blot analysis of extracts from serum-starved U266 and Jurkat cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (50 ng/ml, 15 min; +) using Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb (upper) or total Tyk2 (D4I5T) Rabbit mAb #14193 (lower). |
