| 货号 | 89930S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP,F |
| 目标/特异性 | Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb recognizes endogenous levels of SAMDH1 protein only when phosphorylated at Thr592. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) Flow Cytometry (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SAM domain and HD domain-containing protein 1 (SAMHD1) is a negative regulator of the cell-intrinsic innate immune response (1). Research studies have identified mutations in SAMHD1 as a cause of Aicardi-Goutieres syndrome, an autoimmune disease characterized by elevated production of interferon-α and symptoms resembling congenital viral infection (1). SAMHD1 was identified as the restriction factor that renders most myeloid cells refractory to human immunodeficiency virus (HIV) infection (2-4). Expression of the viral protein Vpx in refractory cells targets SAMHD1 for ubiquitin-mediated degradation and relieves HIV restriction. SAMHD1 prevents autoimmunity and HIV infection by hydrolyzing intracellular deoxynucleoside triphosphates (dNTPs), thereby limiting inappropriate immune activation by self nucleic acid and inhibiting reverse transcription of the HIV genome (4-6). Phosphorylation of Thr592 by cyclin A2/CDK1 was identified as a regulatory mechanism that controls SAMHD1 activity (7,8). SAMHD1 is phosphorylated in proliferating cells, which inhibits its ability to block HIV infection. In resting cells or in cells treated with PMA (TPA) or IFN-α, SAMHD1 phosphorylation is decreased and cells are refractory to HIV infection (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 72 |
| 参考文献 | 1 . Rice, G.I. et al. (2009) Nat Genet 41, 829-32. 2 . Laguette, N. et al. (2011) Nature 474, 654-7. 3 . Hrecka, K. et al. (2011) Nature 474, 658-61. 4 . Powell, R.D. et al. (2011) J Biol Chem 286, 43596-600. 5 . Goldstone, D.C. et al. (2011) Nature 480, 379-82. 6 . Lahouassa, H. et al. (2012) Nat Immunol 13, 223-8. 7 . Cribier, A. et al. (2013) Cell Rep 3, 1036-43. 8 . White, T.E. et al. (2013) Cell Host Microbe 13, 441-51. |
Western blot analysis of extracts from THP-1 cells, untreated (-) or treated with calf intestinal phosphatase (CIP) plus λ phosphatase (+), using Phospho-SAMHD1 (Thr592) (D7O2M) (upper) or SAMHD1 Antibody #12361 (lower). | |
Western blot analysis of extracts from Jurkat and THP-1 cells, untreated (-) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 16 hr; +), using Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb (upper), SAMHD1 Antibody #12361 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of phospho-SAMHD1 (Thr592) from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-SAMDHD1 (Thr592) (D7O2M) Rabbit mAb. Western blot analysis was performed using Phospho-SAMDHD1 (Thr592) (D7O2M) Rabbit mAb. | |
Flow cytometric analysis of THP-1 cells, treated with TPA (80 nM, 16 hr; red) or untreated (green), using Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Flow cytometric analysis of Jurkat cells (blue) and THP-1 cells (green) using Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
