| 货号 | 64616S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Monkey |
| 应用 | WB,IP |
| 目标/特异性 | Phospho-FoxO3a (Ser425) (D5E9T) Rabbit mAb recognizes endogenous levels of FoxO3a protein only when phosphorylated at Ser425. This antibody does not cross-react with FoxO1 nor FoxO4 proteins. This antibody may detect a non-specific band at 65 kDa and at 240 kDa. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). ERK phosphorylates FoxO3a at Ser425, degrading FoxO3a via the ubiquitin-proteasome pathway. Research has shown that this phosphorylation event inhibits FoxO3as function to induce cell death and thus promotes cell proliferation and tumorigenesis (10, 11). |
| 存放说明 | -20C |
| 计算分子量 | 82-97 |
| 参考文献 | 1 . Anderson, M.J. et al. (1998) Genomics 47, 187-99. 2 . Galili, N. et al. (1993) Nat Genet 5, 230-5. 3 . Borkhardt, A. et al. (1997) Oncogene 14, 195-202. 4 . Nakae, J. et al. (1999) J Biol Chem 274, 15982-5. 5 . Rena, G. et al. (1999) J Biol Chem 274, 17179-83. 6 . Guo, S. et al. (1999) J Biol Chem 274, 17184-92. 7 . Seoane, J. et al. (2004) Cell 117, 211-23. 8 . Arden, K.C. (2004) Mol Cell 14, 416-8. 9 . Yang, Y. et al. (2005) EMBO J 24, 1021-32. 10 . Yang JY et al. (2008) Nat Cell Biol 10, 138–48 11 . Brunet, A. et al. (2004) Science 303, 2011-5. |
Western blot analysis of extracts from serum-starved MCF7 cells, untreated (-), or treated (+) with combinations of the following treatments as indicated: stimulated with TPA #4174 (200 nM, 20 min), or pre-treated with U0126 #9903 (10 µM, 2 hr), using Phospho-FoxO3a (Ser425) (D5E9T) Rabbit mAb (upper) or FoxO3a (75D8) Rabbit mAb #2497 (lower). |
