| 货号 | 39357S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB |
| 目标/特异性 | Phospho-Tau (Ser202) (D4H7E) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Ser202. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimers disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Investigators have shown that Tau is phosphorylated during development and hyperphosphorylated at Ser202 in Alzheimers disease (4). |
| 存放说明 | -20C |
| 计算分子量 | 50-80 |
| 参考文献 | 1 . Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9. 2 . Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76. 3 . Bramblett, G.T. et al. (1993) Neuron 10, 1089-99. 4 . Goedert, M. et al. (1993) Proc Natl Acad Sci U S A 90, 5066-70. |
Western blot analysis of extracts from neonatal and adult mouse brain using Phospho-Tau (Ser202) (D4H7E) Rabbit mAb. The phospho-specificity of the antibody was verified by blocking with a phospho- or nonphosphopeptide. | |
Western blot analysis of extracts from mouse brain, untreated (-) or λ-phosphatase-treated (+), using Phospho-Tau (Ser202) (D4H7E) Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower). |
