| 货号 | 35834S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IHC |
| 目标/特异性 | Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC preferred) recognizes endogenous levels of tau protein when phosphorylated at Ser404. This antibody detects single phosphorylation at Ser404, dual phosphorylation at Ser400/Ser404 or Thr403/Ser404, and triple phosphorylation at Ser400/Thr403/Ser404. This antibody does not detect peptides with single phosphorylation at Ser400 or Thr403, and dual phosphorylation at Ser400/Thr403. |
| 使用方法 | Western Blotting (1:1000) Immunohistochemistry (Paraffin) (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimers disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Investigators have shown that tau phosphorylation at Ser404 destabilizes microtubules and that tau is hyperphosphorylated at Ser404 in Alzheimers disease (4-7). |
| 存放说明 | -20C |
| 计算分子量 | 50-80 |
| 参考文献 | 1 . Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9. 2 . Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76. 3 . Bramblett, G.T. et al. (1993) Neuron 10, 1089-99. 4 . Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76. 5 . Shiurba, R.A. et al. (1996) Brain Res 737, 119-32. 6 . Evans, D.B. et al. (2000) J Biol Chem 275, 24977-83. 7 . Bertrand, J. et al. (2010) Neuroscience 168, 323-34. |
Immunohistochemical analysis of paraffin-embedded mouse colon, untreated (left) or λ phosphatase-treated (right), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC Preferred). | |
Western blot analysis of extracts from human cortex (lane 1), untreated neonatal mouse brain (lane 2), phosphatase-treated neonatal mouse brain (lane 3), and fetal rat brain (lane 4), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human Alzheimers brain using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC Preferred). | |
Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC Preferred) in the presence of non-phosphorylated tau peptide (left) and antigen-specific phospho-tau (Ser404) peptide (right). | |
Immunohistochemical analysis of paraffin-embedded T-47D cell pellets (left, positive) and MDA-MB-231 cell pellets (right, negative) using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC Preferred). |
