| 货号 | 75574S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse |
| 应用 | WB,IP |
| 目标/特异性 | Phospho-AMPA Receptor 1 (GluA1) (Ser831) (A5O2P) Rabbit mAb recognizes endogenous levels of AMPA Receptor 1 (GluA1) protein only when phosphorylated Ser831. While the literature refers to this residue as Ser831, it is Ser849 in the UniProt sequence P42261. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluR 2-containing AMPARs, AMPARs that lack GluR 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1). AMPA-type glutamate receptor activity is regulated by phosphorylation, which plays an important role in synaptic plasticity. CaMKII and PKC phosphorylate GluR 1 at Ser831, while PKA phosphorylates GluR 1 at Ser845 (3-5). Furthermore, Ser845 phosphorylation is increased by activation of D1-type dopamine receptors and by inhibition of protein phosphatase 1/protein phosphatase 2A (5,6). Phosphorylation at either Ser831 or Ser845 potentiates AMPA receptor ion channel function: long-term potentiation (LTP) correlates with increased phosphorylation, while long-term depression (LTD) correlates with a dephosphorylation of GluR 1 (6). Phosphomutant mice (Ser831Ala and Ser845Ala) show deficits in LTD and LTP. Either Ser831 or Ser845 alone may support LTP, while only Ser845 is critical for LTD. Furthermore, these mice have memory deficiencies in spatial learning tasks (7,8). GluR 1 receptors are phosphorylated at either Ser831 or Ser845 at ~15-20% under basal conditions and ~50% under stimulated conditions (behavioral or pharmacological) (9). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77. 2 . Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97. 3 . Mammen, A.L. et al. (1997) J Biol Chem 272, 32528-33. 4 . Barria, A. et al. (1997) J Biol Chem 272, 32727-30. 5 . Diering, G.H. et al. (2016) Proc Natl Acad Sci U S A 113, E4920-7. 6 . Roche, K.W. et al. (1996) Neuron 16, 1179-88. 7 . Lee, H.K. et al. (2000) Nature 405, 955-9. 8 . Lee, H.K. et al. (2003) Cell 112, 631-43. 9 . He, K. et al. (2009) Proc Natl Acad Sci U S A 106, 20033-8. |
Western blot analysis of extracts from mouse brain, untreated (-) or λ-phosphatase-treated (+), using Phospho-AMPA Receptor 1 (GluA1) (Ser831) (A5O2P) Rabbit mAb (upper) and AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb #13185 (lower). |
