Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb【科瑞奥博】

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Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb

货号： 12629S 基本售价： 3980.0 元规格： -

产品信息

概述
货号	12629S
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W/IP
目标/特异性	Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb recognizes overexpressed levels of NF-κB p65 protein only when acetylated at Lys310.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
背景	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
存放说明	-20C
计算分子量	65

参考图片

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged human NF-κB p65 (hNF-κB p65; +) and HA-tagged human p300 (hp300-HA; +), using Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (upper) or NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (lower).Western blot分析293T细胞的细胞提取物，对照转染(-)或转染表达Myc/DDK标签的人源NF-κB p65 (hNF-κB p65; +)的构建质粒以及HA标签的人源p300 (hp300-HA; +)构建质粒；使用抗体是Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (上图) 或 NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (下图)。

Immunoprecipitation of acetyl-NF-κB p65 (Lys310) from 293T cells, cotransfected with Myc/DDK-tagged human NF-κB p65 and HA-tagged human p300, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb.对293T细胞中的acetyl-NF-κB p65 (Lys310)进行免疫共沉淀，共转染Myc/DDK标签的人源NF-κB p65 和HA标签的人源p300，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或 Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb (泳道3)。泳道1为10%的加入量。Western blot分析使用的抗体是Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb。