| 货号 | 9030S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb recognizes endogenous levels of β-catenin protein only when acetylated at Lys49. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | -20C |
| 计算分子量 | 92 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing CBP (+), using Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb (upper), β-Catenin (D10A8) XP® Rabbit mAb #8480 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). 空载体转染（-）或转染了CBP表达质粒（+）的293T细胞，使用Acetyl-β-Catenin (Lys49) (D7C2)Rabbit mAb（上），β-Catenin (D10A8) XP® Rabbit mAb#8480（中）和β-Actin (D6A8)Rabbit mAb #8457（下）对细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb (upper) or β-Catenin (D10A8) XP® Rabbit mAb #8480 (lower). 使用Acetyl-β-Catenin (Lys49) (D7C2)Rabbit mAb（上）或β-Catenin (D10A8) XP® Rabbit mAb #8480（下）对多种细胞提取物进行western blot分析。 |
