| 货号 | 14702S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 87 (Akt-GFP) |
Confocal immunofluorescent analysis of 293T cells transiently transfected with GFP-tagged wild-type Akt1 (upper left), GFP-tagged mutant E17K Akt1 (upper right), GFP-tagged wild-type Akt2 (lower left), or GFP-tagged mutant E17K Akt2 (lower right) using Akt (E17K Mutant Specific) (D1T7P) Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note: Green - GFP-tagged wild-type Akt and E17K mutant; Red - Akt (E17K mutant); Yellow - overlay of GFP and Akt (E17K mutant). | |
Western blot analysis of extracts from 293T cells, untransfected (lane 1) or transfected with GFP tagged wild-type Akt1 (lane 2), E17K mutant Akt1 (lane 3), wild-type Akt2 (lane 4), or mutant E17K Akt2 (lane 5), using Akt (E17K Mutant Specific) (D1T7P) Rabbit mAb (upper) and GFP (D5.1) XP® Rabbit mAb #2956 (lower). | |
Immunoprecipitation of Akt (E17K) from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Akt (E17K Mutant Specific) (D1T7P) Rabbit mAb. Western blot analysis was performed using Akt (E17K Mutant Specific) (D1T7P) Rabbit mAb. |
