Asymmetric Di-Methyl Arginine Motif [adme-R] MultiMab™ Rabbit mAb mix
货号：
13522S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 13522S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Arginine methylation is a prevalent PTM found on both nuclear and cytoplasmic proteins. Arginine methylated proteins are involved in many different cellular processes, including transcriptional regulation, signal transduction, RNA metabolism, and DNA damage repair (1-3). Arginine methylation is carried out by the arginine N-methyltransferase (PRMT) family of enzymes that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (4). There are three different types of arginine methylation: asymmetric dimethylarginine (aDMA, omega-NG,NG-dimethylarginine), where two methyl groups are placed on one of the terminal nitrogen atoms of the guanidine group of arginine; symmetric dimethylarginine (sDMA, omega-NG,N’G-dimethylarginine), where one methyl group is placed on each of the two terminal guanidine nitrogens of arginine; and monomethylarginine (MMA, omega-NG-dimethylarginine), where a single methyl group is placed on one of the terminal nitrogen atoms of arginine. Each of these modifications has potentially different functional consequences. Though all PRMT proteins catalyze the formation of MMA, Type I PRMTs (PRMT1, 3, 4, and 6) add an additional methyl group to produce aDMA, while Type II PRMTs (PRMT5 and 7) produce sDMA. Methylated arginine residues often reside in glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (5). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within proline-glycine-methionine rich (PGM) motifs (6). |
| 存放说明 | -20C |
参考图片Western blot analysis of MCF7 cells, untreated (-) or treated with Adenosine-2, 3-dialdehyde (AdOx, 100 μM, 24 hr; +), using Asymmetric Di-Methyl Arginine Motif [adme-R] Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). |
The specificity of Asymmetric Di-Methyl Arginine Motif [adme-R] was determined using peptide ELISA. The figure demonstrates that the antibody is specific for asymmetric di-methyl arginine and does not react with mono-methyl, di-methyl or tri-methyl lysine and does not react with mono-methyl or symmetric di-methyl arginine. |
