Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab™ Rabbit mAb mix
货号：
8698S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 8698S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Cleaved Caspase Substrate Motif [DE(T/S/A)D] Rabbit mAb recognizes endogenous levels of caspase-cleaved proteins with a carboxy-terminal aspartic acid residue, and in rare cases a carboxy-terminal glutamic acid residue . This antibody does not cross-react with whole proteins or those ending with a different carboxy-terminal residue. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Apoptosis is a physiological process resulting in a highly regulated, programmed form of cell death that is a normal part of growth and development in multicellular organisms. Caspases are aspartic acid-directed cysteine proteases that are central to the apoptotic mechanism (1). The intrinsic pathway initiates an apoptotic cascade from signals originating within the cell, such as DNA damage, while an extrinsic pathway initiates apoptosis in response to extracellular signals, like FasL. In both intrinsic and extrinsic pathways, initiator caspases cleave downstream substrates, including multiple effector caspases and the primary executioner of cell death, caspase-3 (2,3). Effector caspases amplify the apoptotic cascade to target many critical proteins needed for normal cell function. Apart from its role in developmental biology, the regulation of apoptosis has broad implications for the study of cancer, autoimmune disorders and infectious diseases (4). Thousands of known and putative caspase cleavage sites are present within the human proteome; almost all sites involve cleavage at an aspartic acid residue, though cleavage at glutamic acid residues is seen, rarely, as well (5). |
| 存放说明 | -20C |
参考图片Western blot analysis of NIH/3T3 and HeLa cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +), using Cleaved Caspase Substrate Motif [DE(T/S/A)D] Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 as a loading control (lower). Western blot方法分别检测未处理（-）或经Staurosporine #9953 (1 μM, 3 hr; +)处理的NIH/3T3 和HeLa细胞提取物，使用的抗体为Cleaved Caspase Substrate Motif [DE(T/S/A)D] Rabbit mAb (上图)， GAPDH (D16H11) XP® Rabbit mAb #5174 作为上样量对照 (下图)。 |
