| 货号 | 9579S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | IHC-P/IF-IC/F |
| 目标/特异性 | Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. This antibody is preferred for immunfluorescence, flow cytometry, and immunohistochemistry applications. This antibody detects non-specific caspase substrates by western blot. |
| 使用方法 | IHC-P(1:250) F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | -20C |
Immunohistochemical analysis of paraffin-embedded HT-29 xenograft using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right). 采用Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗对石蜡包埋的HT-29异种移植体，进行免疫组化分析(IHC)，抗体孵育时，左图采用对照多肽，右图采用Cleaved Caspase-3 (Asp175) Blocking Peptide #1050。 | |
Immunohistochemical analysis of paraffin-embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb on SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104. 免疫组化（IHC）分析石蜡包埋的Jurkat细胞颗粒，未处理（左图）或etoposide （依托泊苷）（右图）处理，抗体采用Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗，并使用 SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls #8104。 | |
Immunohistochemical analysis of paraffin-embedded human malignant stromal tumor using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb. | |
采用Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗对石蜡包埋的人恶性基质瘤进行免疫组化分析（IHC）。 |
