| 货号 | 7237S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb recognizes endogenous levels of caspase-9 protein only when cleaved at Asp330. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr) or etoposide (25 μM, overnight), using Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb (upper) or total Caspase-9 Antibody (Human Specific) #9502 (lower). Western免疫印迹。用Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 兔单抗(上图), 或者total Caspase-9 Antibody (Human Specific) #9502 (下图)研究未经处理或经 Staurosporine #9953 (1 μM, 3 hr) 或者etoposide (25 μM, 过夜)处理的HeLa 和Jurkat 细胞的细胞提取液。 |
