| 货号 | 2901S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb detects endogenous levels of histone H3 only when di-methylated on Lys36. The antibody does not cross-react with non-methylated, mono-methylated, or tri-methylated Lys36. In addition, the antibody does not cross-react with di-methylated histone H3 Lys4, Lys9, Lys27, Lys79 or methylated histone H4 Lys20. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Confocal immunofluorescent analysis of HeLa cells using Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb兔单抗 (绿色)，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb. 使用Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb兔单抗，免疫印迹(Western blot)分析不同细胞中Di-Methyl-Histone H3 (Lys36)的蛋白水平。 | |
Antibody specificity was determined by Western blotting. HeLa and NIH/3T3 cell extracts were probed with Di-Methyl Histone H3 (Lys36) (C75H12) Rabbit mAb alone (Panel A) or Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb pre-adsorbed with 1.5 μM of various competitor peptides (Panels B-I). As shown, only the di-methyl-histone H3 (Lys36) peptide competed away binding of the antibody. 抗体特异性通过免疫印迹(Western blot)确定。HeLa和NIH/3T3细胞提取物的探针使用只有Di-Methyl Histone H3 (Lys36) (C75H12) Rabbit mAb兔单抗(图A)或Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb兔单抗，并且其提前孵育了1.5 μM various competitor peptides (图B-I)。如图所示，仅di-methyl-histone H3 (Lys36) peptide竞争离开该抗体的结合。 | |
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb in the presence of non-methyl peptide (left) or K36 di-methyl peptide (right). 使用Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb兔单抗免疫组化分析人源胃癌组织石蜡切片，左图： 非甲基化肽段，右图：K36 双甲基化肽段。 |
