| 货号 | 8015S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Mono-Methyl Arginine (Me-R4-100) Rabbit mAb recognizes endogenous levels of mono-methyl arginine protein. This is a general mono-methyl arginine motif antibody without sequence preferences. It does not cross-react with di-methyl arginine or unmethylated arginine. |
| 使用方法 | WB(1:1000) IP (1:50) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Arginine methylation is a prevalent post-translational modification found on both nuclear and cytoplasmic proteins. Arginine methylated proteins are involved in many different cellular processes, including transcriptional regulation, signal transduction, RNA metabolism, and DNA damage repair (1-3). Arginine methylation is carried out by the arginine N-methyltransferase (PRMT) family of enzymes that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (4). There are three different types of arginine methylation: asymmetric dimethylarginine (aDMA, omega-NG,NG-dimethylarginine), where two methyl groups are placed on one of the terminal nitrogen atoms of the guanidine group of arginine; symmetric dimethylarginine (sDMA, omega-NG,N’G-dimethylarginine), where one methyl group is placed on each of the two terminal guanidine nitrogens of arginine; and monomethylarginine (MMA, omega-NG-dimethylarginine), where a single methyl group is placed on one of the terminal nitrogen atoms of arginine. Each of these modifications has potentially different functional consequences. Though all PRMT proteins catalyze the formation of MMA, Type I PRMTs (PRMT1, 3, 4, and 6) add an additional methyl group to produce aDMA, while Type II PRMTs (PRMT5 and 7) produce sDMA. Methylated arginine residues often reside in glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (5). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within proline-glycine-methionine rich (PGM) motifs (6). |
| 存放说明 | -20C |
Western blot analysis of extracts from HCT 116 cells using Mono-Methyl Arginine (Me-R4-100) Rabbit mAb. Specific signals are blocked by mono-methyl arginine antigen peptide, but not by non-methyl arginine control peptide. 对HCT116细胞抽提液使用Mono-Methyl Arginine (Me-R4-100) Rabbit mAb进行Western blot分析。特异性信号被单甲基化的精氨酸抗原肽段阻断，但是不能被非甲基化的精氨酸对照肽段阻断。 | |
Western blot analysis of extracts from various mouse tissues using Mono-Methyl Arginine (Me-R4-100) Rabbit mAb. 对多个小鼠组织使用Mono-Methyl Arginine (Me-R4-100) Rabbit mAb进行Western blot分析。 |
