| 货号 | 9477S |
| 反应种属 | Human/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb recognizes phospho-serine in a KS*P motif. The antibody does not cross-react with phospho-threonine or phospho-tyrosine containing peptides/proteins. |
| 使用方法 | WB(1:1000) IP (1:50) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-(Ser) CDKs Substrate Antibody recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization, as well as HTS drug screening for potential kinase regulators. |
| 存放说明 | -20C |
Western blot analysis of extracts from COS-7 cells, synchronized in G1/S, G2/M, and G0 phase of the cell cycle, using Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb. | |
对COS-7细胞，将其同步在G1/S、G2/M和G0期，使用Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb兔单抗进行Western blot分析其蛋白抽提物。 |
