| 货号 | 6967S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb detects endogenous levels of many cellular proteins only when phosphorylated at Ser residues surrounded by Arg or Lys at the -2 and +2 positions. The antibody may also cross-react with S*X(R/K) motif-containing proteins. The antibody does not cross-react with nonphosphorylated Ser residues, phospho-Thr in the same motif, or phospho-Ser in other motifs. |
| 使用方法 | WB(1:1000) IP (1:50) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction (1,2), many of their substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). To identify potential PKC substrates, Cell Signaling Technology has developed antibodies that recognize this motif. Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb is specific to cPKC substrates containing phospho-Ser. |
| 存放说明 | -20C |
Western blot analysis of extracts from Jurkat cells, untreated (-) or TPA-treated (200 nM, 30 min; +), using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biotechnologies). 对未处理（-）或200nM TPA处理30分钟（+）的Jurkat细胞抽提液使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnologies)。 | |
Immunoprecipitation (IP) of extracts from Jurkat cells, untreated (-) or TPA-treated (200 nM, 30 min; +) (lanes 3 and 4), using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb. 10% input is shown in lanes 1 and 2. Western blot analysis was performed using the same antibody. 对未处理（-）或200nM TPA处理30分钟的（+）（列3、4）的Jurkat细胞，使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb兔单抗进行免疫沉淀。10%输入对照显示在列1和2。Western Blot分析用相同抗体进行。 | |
Western blot analysis of extracts from various mouse tissues using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb. 对多种小鼠组织抽提液使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb兔单抗进行Western blot分析。 |
