| 货号 | 5759S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also cross-reacts with proteins and peptides that only harbor an RXXpS/pT motif. |
| 使用方法 | WB(1:1000) IP (1:100) E-P (1:1000) |
| 供应商 | CST |
| 背景 | AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The β1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the β1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1). |
| 存放说明 | -20C |
Western blot analysis of extracts from H1650 cells, untreated or treated with phenformin (5 mM, 1 hr), using Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biosciences). 对未处理（-）或phenformin（5 mM，1小时，+）处理的H1650细胞抽提液使用Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb兔单抗进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biosciences)。 | |
Western blot analysis of extracts from various mouse tissues using Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb. 对多种小鼠组织抽提液使用Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb兔单抗进行Western blot分析。 | |
Immunoprecipitation of extracts from H1650 cells using Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb. Lane 1 shows 10% input. Western blot analysis was performed using the same antibody. 对H1650细胞抽提液使用Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb兔单抗进行Western blot分析。列1显示10%输入对照。Western Blot分析用相同抗体进行。 |
