Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb
货号：
2909S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2909S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb detects endogenous levels of proteins containing the ATM/ATR substrate motif. This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif. |
| 存放说明 | -20C |
参考图片Western blot analysis of extracts from HeLa cells, untreated or treated with γ-irradiation, using Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb. 对未处理（-）或γ-irradiation处理的HeLa细胞抽提液使用Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb进行Western blot分析。 |
