| 货号 | 8738S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb recognizes endogenous proteins containing a pS/pTDXE motif, which is a CK2 phosphorylation consensus sequence. This antibody is a useful tool to study CK2 substrates. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Casein Kinase II (CK2) is a highly conserved, ubiquitously expressed, and constitutively active tetrameric Ser/Thr protein kinase with hundreds of substrates participating in the regulation of a variety of cellular processes including cell cycle progression, apoptosis, transcription, inflammation, and the DNA damage response. Research studies have implicated CK2 in roles related to viral infection, cancer, and other diseases (1-5). CK2 substrates contain multiple acidic residues (Asp and Glu) located downstream of the phosphorylated Ser or Thr residue. The consensus sequence for CK2 substrates is pS/pTD/EXD/E with the most crucial residue at the +3 position followed by the residue at the +1 position (6). |
| 存放说明 | -20C |
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902 (10 nM, 30 min) or Staurosporine #9953 (1 μM, 3 hr), using Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb. Western blot方法分别检测未处理或经Calyculin A #9902 (10 nM)处理,30 min 或 Staurosporine #9953 (1 μM)处理3 hr的HeLa细胞提取物，使用的抗体为Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb。 | |
Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® CK2α siRNA I #6389 (+), using Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb (upper), CK2α Antibody #2656 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Western blot方法分别检测经SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence® CK2α siRNA I #6389 (+)转染的HeLa细胞提取物，使用的抗体为Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb (上图), CK2α Antibody #2656 (中图), 和 GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。 |
