| 货号 | 9634S |
| 反应种属 | Human/Mouse/All |
| 来源宿主 | Mouse |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb detects phosphorylated serine or threonine that is surrounded by tyrosine or phenylalanine at the -1 and +1 positions and phenylalanine at the -4 position. It also recognizes peptides containing lysine instead of phenylalanine at the -4 position. This antibody does not cross-react with the nonphosphorylated PDK1 docking motifs or with other phosphorylated motifs. This antibody detects endogenous levels of phosphorylated proteins containing PDK1 docking motif, including phospho-Akt. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:20) E-P (1:1000) |
| 供应商 | CST |
| 背景 | A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases (1), but also those that are recognized by phosphorylation-dependent binding proteins such as 14-3-3 (2). These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. CST has pioneered the development of phospho-motif specific antibodies, which are invaluable tools for probing the complexity of phospho-regulatory pathways. |
| 存放说明 | -20C |
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated (0.1 µM for 30 minutes prior to lysis), using Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb. 对未处理或0.1uM calyculin A裂解前处理30分钟的A431细胞抽提液使用Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb进行Western blot分析。 | |
Immunoprecipitation of extracts from NIH/3T3 cells, untreated or treated with 100 ng/ml PDGF for 20 minutes prior to lysis, using Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb and Akt Antibody #9272. Western blots were performed using Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb (upper) or Akt Antibody #9272 (lower). 对未处理或100 ng/ml PDGF裂解前处理20分钟的NIH/3T3细胞抽提液使用Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb and Akt Antibody #9272进行Western blot分析。使用Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb（上）或Akt Antibody #9272（下）进行Western blot分析。 | |
Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb ELISA分析：磷酸化和非磷酸化的信噪比。（T*和S*代表磷酸化的苏氨酸和丝氨酸） |
