| 货号 | 10001S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/E-P |
| 目标/特异性 | Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb recognizes endogenous proteins containing phospho-Ser/Thr preceded by Arg at positions -5 and -3 in a manner largely independent of the surrounding amino acid sequence. Minor cross-reactivity is observed for proteins that contain phospho-Ser/Thr preceded by Arg at position -3 only. No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. |
| 使用方法 | WB(1:1000) E-P (1:1000) |
| 供应商 | CST |
| 背景 | An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. |
| 存放说明 | -20C |
Western blot analysis of extracts from MKN-45 cells, untreated or treated with c-Met kinase inhibitor SU11274 (1 µM, 2 hr), using Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb. Western blot image was obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology). 对未处理（-）或c-Met激酶抑制剂SU11274（1 uM，2小时）处理的MKN-45细胞抽提液使用Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb兔单抗进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnology)。 | |
Western blot analysis of extracts from A-431 cells, untreated or treated with Human Epidermal Growth Factor (hEGF) #8916 (10 ng/ml, 20 min), using Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb (upper), or Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (lower). Western blot images were obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology). 对未处理（-）或人表皮生长因子(hEGF) #8916 (10 ng/ml, 20 min)处理的A-431细胞抽提液使用Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb（上）和Phospho-Akt (Ser473) (D9E) XP庐 Rabbit mAb #4060（下）兔单抗进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnology)。 |
