| 货号 | 12127S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb recognizes endogenous levels of ALK only when phosphorylated at Tyr1078, which is equivalent to Tyr138 of NPM-ALK. This antibody may cross-react weakly with other overexpressed phospho-tyrosine kinases such as EGFR and Src. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 220 (ALK), 80 (NPM-ALK) |
Western blot analysis of extracts from KARPAS-299 cells, untreated (-) or treated with calf intestinal phosphatase (CIP; +), using Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb (upper) or ALK (C26G7) Rabbit mAb #3333 (lower).Western blot方法检测KARPAS-299细胞提取物，细胞未处理（-）或用小牛肠磷酸酶(CIP; +)处理，使用的抗体为Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb (上图)或ALK (C26G7) Rabbit mAb #3333 (下图)。 | |
Immunoprecipitation of phospho-NPM-ALK(Tyr138) from KARPAS-299 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb.免疫沉淀方法检测KARPAS-299细胞提取物中的磷酸化-NPM-ALK(Tyr138)，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb (泳道3)。泳道1是加入10%蛋白。用Phospho-ALK (Tyr1078) (D28B4) Rabbit mAb进行Western blot分析。 |
