Phospho-ALK (Tyr1586) (3B4) Rabbit mAb
货号：
3348S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3348S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and EML4 has been described from a non-small cell lung cancer cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 80 (NPM-ALK) 220 (ALK) |
参考图片Western blot analysis of Sup-M2 cell lysate, using Phospho-ALK (Tyr1586) (3B4) Rabbit mAb (A and B), and ALK Antibody (C and D).The phospho-specificity of this antibody was characterized by treating the membrane with calf intestinal alkaline phosphatase (CIP) (B and D) after Western transfer.用抗磷酸化的ALK (Tyr1586)的兔源单克隆抗体(3B4)（A, B）或抗ALK的抗体（C, D）对Sup-M2细胞提取物进行蛋白质印记检测。转膜后用小牛肠碱性磷酸酶（CIP）对膜进行处理（B, D），可以检测磷酸化抗体的特异性。 |
