| 货号 | 9607S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-ATM/ATR Substrate Motif (S*Q) (D23H2/D69H5) Rabbit mAb recognizes peptides and proteins containing sequences of phospho-Ser followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser containing motifs. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif. |
| 存放说明 | -20C |
Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (+, 2 hr), using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biotechnologies). 对未处理（-）或UV（两小时，+）处理30分钟的HeLa细胞抽提液使用Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnologies)。 | |
Immunoprecipitation of HeLa cells, untreated (-) or UV-treated (+, 2 hr) (lanes 3 and 4), using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb. 10% input is shown in lanes 1 and 2. Western blot analysis was performed using the same antibody (upper) and Chk1 (2G1D5) Mouse mAb #2360 (lower). 对未处理（-）或UV（两小时，+）处理（列3和列4）的HeLa细胞抽提液使用Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb进行免疫共沉淀分析。10%输入显示在列1和2。Western Blot使用相同抗体（上）和Chk1 (2G1D5) Mouse mAb #2360（下）。 |
