Phospho-Bad (Ser136) (185D10) Rabbit mAb
货号：
5286S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 5286S |
| 反应种属 | Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Bad (Ser136) (185D10) Rabbit mAb detects transfected levels of Bad protein only when phosphorylated at Ser136. |
| 使用方法 | WB(1:500) |
性能| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
参考图片Western blot analysis of extracts from control 293, GST-Bad transfected 293 and COS cells, untreated or TPA-treated, using Phospho-Bad (Ser136) (185D10) Rabbit mAb.Western blot免疫印迹分析对照293细胞，GST-Bad转染的293细胞和COS细胞的细胞提取液，未经过处理和经过TPA处理的，所用抗体为Phospho-Bad (Ser136) (185D10) Rabbit mAb 兔单抗。 |
