| 货号 | 4366S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Bad (Ser136) (D25H8) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser136 (equivalent to Ser99 in human and Ser137 in rat). |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Western blot analysis of extracts from ACHN cells, untreated or treated with Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min), and C2C12 cells, untreated or insulin-treated (100 nM, 30 min), using Phospho-Bad (Ser136) (D25H8) Rabbit mAb (upper) or Bad (D24A9) Rabbit mAb #9239 (lower). Western blot免疫印迹分析未处理和经Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min)处理的 ACHN 细胞的细胞提取液，未处理和经insulin处理(100 nM, 30 min)的C2C12细胞的细胞提取液，用Phospho-Bad (Ser136) (D25H8) Rabbit mAb 兔单抗 (上图)或者Bad (D24A9) Rabbit mAb 兔单抗#9239 (下图)研究。 | |
Western blot analysis of extracts from COS-7 cells, treated with Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min) in the presence or absence of calf intestinal phosphatase (CIP) plus lambda-phosphatase, using Phospho-Bad (Ser136) (D25H8) Rabbit mAb (upper) or Bad (D24A9) Rabbit mAb #9239 (lower). Western blot免疫印迹分析细胞裂解液，在小牛肠碱性磷酸酶(CIP)和 lambda 磷酸酶存在或者不存在下，用Human Epidermal Growth Factor #8916 (100 ng/ml, 30 min)处理COS-7细胞， 所用抗体为Phospho-Bad (Ser136) (D25H8) Rabbit mAb 兔单抗(上图)或者Bad (D24A9) Rabbit mAb 兔单抗#9239 (下图)。 | |
