| 货号 | 14717S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | To induce autophagy, AMP-activated protein kinase (AMPK) directly phosphorylates Beclin-1 at conserved Ser93 and Ser96 residues in human, which correspond to murine Ser91 and Ser94 (8). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Immunoprecipitation of phospho-Beclin-1 (Ser93) from carbonyl cyanide 3-chlorophenyldydrazone (CCCP)-treated KARPAS-299 cells (100 μM, 2 hr) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross reactivity with IgG heavy chain. Cell line source: Dr Abraham Karpas at the University of Cambridge. | |
Western blot analysis of extracts from MCF7 and KARPAS-299 cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhdrazone (CCCP, 100 μM, 2 hr; +), using Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb (upper) and Beclin-1 (D40C5) Rabbit mAb #3495 (lower). Cell line source: Dr Abraham Karpas at the University of Cambridge. | |
Western blot analysis of extracts from 293T cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 100 μM, 2 hr; +) and mock transfected (-) or transfected with a construct expressing full-length human Beclin-1 (hBeclin-1; +), using Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb (upper) and Beclin-1 (D40C5) Rabbit mAb #3495 (lower). |
