| 货号 | 12433S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Bim (Ser77) (D4H12) Rabbit mAb recognizes endogenous levels of Bim protein only when phosphorylated at Ser77. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk, and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1, and BHRF-1 (1,2). Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL, and BimS (1). The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity. |
| 存放说明 | -20C |
| 计算分子量 | 26 |
Western blot analysis of extracts from Raji and MCF7 cells, untreated (-) or treated with TPA #4174 (200 nM, 30 min; +), using Phospho-Bim (Ser77) (D4H12) Rabbit mAb (upper) or Bim (C34C5) Rabbit mAb #2933 (lower). Western blot 检测 Raji 和 MCF7细胞提取物，未处理 (-)或TPA #4174 (200 nM, 30 min; +)处理，使用抗体为Phospho-Bim (Ser77) (D4H12) Rabbit mAb 兔单抗（上图）或Bim (C34C5) Rabbit mAb 兔单抗#2933（下图）。 | |
