Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb
货号：
2868S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2868S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 135 (c-Abl); 210 (Bcr-Abl) |
参考图片Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins, using Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).western blot方法检测细胞提取物：表达各种有活性酪氨酸激酶蛋白的细胞，使用的抗体是Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb (上图)和 Phospho-Tyrosine mAb (P-Tyr-100) #9411 (下图）。 |
Western blot analysis of CML-T1 and BV173 cell lysates, using Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane with (+) (right) or without (-) (left) calf intestinal phosphatase (CIP) after Western transfer. |
