Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb
货号：
3098S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3098S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 135 (c-Abl); 210 (Bcr-Abl) |
参考图片Western blot analysis of extract from CML-T1 leukemic cells with Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb (upper and middle) or c-Abl Antibody #2862 (lower). The phospho-specificity of this rabbit mAb was verified by treating the membrane with calf intestinal phosphatase (CIP) (middle and lower) before antibody probing.western blot方法检测细胞提取物：用Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb (上图和中图)和c-Abl Antibody #2862 (下图)处理的CML-T1白血病细胞。在使用抗体检测之前，先用牛小肠碱性磷酸酶处理转印膜已验证这种特异位点磷酸化的兔单克隆抗体。 |
