| 货号 | 12716S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb recognizes endogenous levels of CamKII-α protein only when phosphorylated at Thr286. This antibody also recognizes endogenous levels of CamKII-β and CamKII-γ protein only when phosphorylated at Thr287. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 60, 50 |
Western blot analysis of extracts from 293T cells, untransfected or transfected with specific CamKII proteins as indicated, using Phospho-CamKII (Thr286) (D21E4) Rabbit mAb. Antibody phosphospecificity was verified by preincubating the antibody in the absence of a peptide (-) or with either CamKII-β (Thr287) phosphopeptide (+) or CamKII-β (Thr287) non-phosphopeptide (+) prior to incubating the membrane. | |
Western blot分析未转染或转染了特异的CamKII蛋白的293T细胞提取物，使用的抗体是Phospho-CamKII (Thr286) (D21E4) Rabbit mAb 兔单抗。通过将抗体与对照(-)或CamKII-β (Thr287) phosphopeptide (+) 或 CamKII-β (Thr287) non-phosphopeptide (+)预孵育，再与膜孵育，以确定抗体的磷酸化特异性。 | |
Western blot analysis of extracts from rat brain, mouse brain and human cerebellum using Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb. Western blot分析大鼠大脑，小鼠大脑和人小脑提取物，使用的抗体是Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb 兔单抗。 |
