| 货号 | 4901S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活，该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化，而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时，cdc25C结合14-3-3蛋白家族的成员，扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。 |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.免疫组织化学方法检测石蜡包埋人类结肠癌组织，显示胞浆定位，使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.免疫组织化学分析石蜡包埋人类乳腺癌组织，使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.免疫组织化学分析石蜡包埋人类肺癌组织，使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.免疫组织化学分析石蜡包埋人类肺癌组织，左图为未处理组织，右图为lambda磷酸酶处理的组织，使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lymph node, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb in the presence of control peptide (left) or Phospho-cdc25C (Ser216) Blocking Peptide #1190 (right).免疫组织化学分析石蜡包埋人类淋巴结组织，左图为对照多肽，右图为Phospho-cdc25C (Ser216) Blocking Peptide 多肽#1190 ，使用的抗体为Phospho-cdc25C (Ser216) (63F9) Rabbit mAb 。 | |
Western blot analysis of GST-cdc25C, unphosphorylated or phosphorylated in vitro by Chk2, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb (upper) or cdc25C antibody #9522 (lower). | |
Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit Mab, #4688, (lower).western blot方法检测未处理、nocodazole处理和λ磷酸酶处理的HT29细胞提取物，使用的抗体为Phospho-cdc25C (Ser216) antibody (上图)和cdc25C (5H9) Rabbit Mab, #4688, (下图)。 |
