| 货号 | 12028S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:300) |
| 供应商 | CST |
| 背景 | Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).Cdc25 是一种蛋白磷酸酯酶，能够去磷酸化激活cdc2，这是对于所有真核细胞进入有丝分裂期这关键一步的调控（1）。在有丝分裂间期，cdc25C的216位丝氨酸会一直被c-TAK1磷酸化，该位点的磷酸化在G2/M检验期依赖于DNA损伤（2）。当216位丝氨酸被磷酸化后，cdc25C可以和14-3-3家族蛋白结合，是cdc25C退回细胞质避免过早的有丝分裂（3）。检验点激酶Chk1和Chk2在DNA损伤后会磷酸化cdc25C的216位丝氨酸（4,5）。 |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb (绿色)对未处理（左）或λ phosphatase处理（右）的HT-29细胞进行激光共聚焦荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HT-29 cells, asynchronous (-) or synchronized in mitosis (thymidine block, nocodazole release, mitotic shake-off; +), using Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb (upper) or cdc25C (5H9) Rabbit mAb #4688 (lower).对细胞周期不同（-）或同步（thymidine block, nocodazole release, mitotic shake-off; +）的HT-29细胞提取物，使用使用Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb（上）或cdc25C（5H9）Rabbit Mab#4688（下）进行western blot分析。 |
