| 货号 | 14371S |
| 反应种属 | Human/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transition through their association and subsequent phosphorylation of substrate proteins in a proline-directed fashion. CDKs phosphorylate a serine or threonine residue within the (S/T)PX(R/K) consensus sequence (1-5). Cell Signaling Technology has developed antibodies to phosphorylated proline-rich motifs for broad-based discovery applications. The TPXK motif is targeted for phosphorylation by CDKs 1, 2, 4, 5, and 6 (1-5). |
| 存放说明 | -20C |
Western blot analysis of extracts from HCT 116 cells, G0 cells that were serum starved and contact inhibited (36 hr) or mitotic cells treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (50 ng/ml, 20 hr), using Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). | |
Immunoprecipitation of phospho-TPXK motif-containing proteins from mitotic HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 or Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb. Lane 1 is 10% input. Western blot analysis was performed using Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb followed by Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 as secondary antibodies. |
