| 货号 | 3401S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb (upper) or PKA RI-α (D54D9) Rabbit mAb #5675 (lower) (left panel). The phospho-specificity of the antibody was verified by blocking with a phospho (middle panel) or nonphosphopeptide (right panel). | |
Western blot analysis of extracts from MKN-45 cells using Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer. |
