Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb
货号：
12438S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 12438S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb detects endogenous levels of DARPP-32 only when phosphorylated at Thr34. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
参考图片Western blot analysis of extracts from MKN-45 cells, untreated (-) or treated with Forskolin #3828 (30 μM, 20 min; +), or nonphospho-DARPP-32 and phospho-DARPP-32 (Thr34) recombinant proteins using Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb (upper) or DARPP-32 (19A3) Rabbit mAb #2306 (lower). Western blot 检测 MKN-45 细胞提取物，未处理 (-)或 Forskolin #3828处理 (30 μM, 20 min; +)或 非磷酸化-DARPP-32及磷酸化-DARPP-32 (Thr34) 重组蛋白，使用的抗体是Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb兔单抗 （上图）或 DARPP-32 (19A3) Rabbit mAb 兔单抗#2306（下图）。 |
