Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb
货号：
8808S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 8808S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb detects endogenous levels of EGFR protein only when phosphorylated at Thr669. While the literature refers to this residue as Thr669, it is Thr693 of human EGFR (UniProt sequence P00533) and corresponds to Thr695 of mouse EGFR or Thr694 of rat EGFR. |
| 使用方法 | WB(1:1000)
IP (1:200) |
性能| 供应商 | CST |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
参考图片Western blot analysis of extracts from A-431 cells serum starved overnight, and left untreated (-) or treated (+) with EGF (100 ng/ml, 15 min), using Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb (upper) and EGF Receptor (D38B1) XP® Rabbit mAb #4267 (lower).Western blot方法检测血清饥饿过夜处理的A-431细胞提取物，剩余细胞不处理（-）或用EGF (100 ng/ml, 15 min)处理（+），使用的抗体为Phospho-EGF Receptor (Thr669) (D2F1) Rabbit mAb (上图)和EGF Receptor (D38B1) XP® Rabbit mAb #4267 (下图). |
