| 货号 | 6347S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb recognizes endogenous levels of EphA2 protein only when phosphorylated at Ser897. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:8000) |
| 供应商 | CST |
| 背景 | The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyr324 and Tyr327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7). |
| 存放说明 | -20C |
| 计算分子量 | 125 |
Western blot analysis of extracts from SNB19 cells, serum starved overnight and left untreated (-) or treated with FBS (10%, 5 min; +), using Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb (upper) or EphA2 (D4A2) XP® Rabbit mAb #6997 (lower).Western blot方法检测SNB19细胞提取物，细胞经血清饥饿过夜后不处理（-）或用FBS (10%, 5 min; +)处理，使用的抗体为Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb (上图)或EphA2 (D4A2) XP® Rabbit mAb #6997 (下图). | |
Immunohistochemical analysis of paraffin-embedded SNB19 cell pellets, control (left) or 10% FBS-treated (right), using Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb.免疫组织化学方法检测石蜡包埋的SNB19细胞球，对照（左图）或10% FBS处理（右图），使用的抗体为Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人卵巢癌组织，对照（左图）或用λ磷酸酶处理（右图），使用的抗体为Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人前列腺癌组织，使用的抗体为Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb. |
