| 货号 | 12677S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb recognizes endogenous levels of EphA2 protein only when phosphorylated at Tyr588. This antibody may cross-react with other overexpressed phosphotyrosine proteins. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyr324 and Tyr327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7). |
| 存放说明 | -20C |
| 计算分子量 | 125 |
Western blot analysis of extracts from SNB19 cells, untreated (-) or EphrinA1-Fc ligand-treated (100 ng/ml, 5 min; +), using Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb (upper) or EphA2 (D4A2) XP® Rabbit mAb #6997 (lower).Western blot方法检测SNB19细胞提取物，细胞不处理（-）或用EphrinA1-Fc配体处理（100 ng/ml, 5 min; +），使用的抗体为Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb (上图)或 EphA2 (D4A2) XP® Rabbit mAb #6997 (下图)。 | |
Immunoprecipitation of phospho-EphA2 (Tyr588) from EphrinA1-Fc ligand-treated (100 ng/ml, 5 min) SNB19 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb.免疫沉淀方法检测EphrinA1-Fc配体(100 ng/ml, 5 min)处理的SNB19细胞提取物中的磷酸化EphA2 (Tyr588)，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb (泳道3)。泳道1是加入10%蛋白。用Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb进行Western blot分析。 |
