Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb
货号：
8862S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 8862S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb recognizes endogenous levels of EphA3 protein only when phosphorylated at Tyr779. This antibody cross-reacts with EphA2, EphA4, and EphA5 at the corresponding phosphosites. |
| 使用方法 | WB(1:1000)
IP (1:100) |
性能| 供应商 | CST |
| 背景 | The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5).
The EphA3 receptor preferentially binds ephrin-A5. This ligand-receptor interaction stimulates EphA3 signaling, regulates cell adhesion and migration, and induces cellular morphologic responses (6-8). EphA3 plays a critical role in callosal axon guidance (9), retinotectal mapping of neurons (10), as well as cardiac cell migration and differentiation (11). Investigators have shown that somatic mutations in functional domains of EphA3 are linked to lung cancer progression (12). In addition, EphA3 expression levels have been correlated with tumor angiogenesis and progression in gastric and colorectal carcinoma (13,14). |
| 存放说明 | -20C |
| 计算分子量 | 135 |
参考图片Western blot analysis of extracts from SNB19 cells, serum starved overnight and either untreated (-) or treated with ephrin-A5-Fc (100 ng/ml, 5 min; +), using Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb (upper) or EphA3/A4/A5 (D2C11) Rabbit mAb #8793 (lower).Western blot方法检测SNB19细胞提取物，细胞经血清饥饿过夜后，不处理（-）或用ephrin-A5-Fc (100 ng/ml, 5 min; +)处理，使用的抗体为Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb (上图)或EphA3/A4/A5 (D2C11) Rabbit mAb #8793 (下图). |
