Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb
货号：
5484S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 5484S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb recognizes endogenous levels of ezrin protein only when phosphorylated at Tyr353. Based on protein sequences, the antibody is not expected to cross-react with radixin or moesin. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
参考图片Western blot analysis of extracts from A-431 cells, untreated or treated with hEGF #8916 (100 ng/ml, 5 minutes), using Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb (upper) or Ezrin/Radixin/Moesin Antibody #3142 (lower).使用Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb (上图)或Ezrin/Radixin/Moesin Antibody #3142 (下图)，免疫印迹（Western Blot）分析A431细胞中Phospho-Ezrin (Tyr353)和Ezrin/Radixin/Moesin蛋白水平，细胞分为未处理组和hEGF #8916 (100 ng/ml, 5 minutes)处理组。 |
