Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb
货号：
2544S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2544S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb detects transfected levels of FGFR-1 only when phosphorylated at tyrosine 766. The antibody may cross-react with other FGFR family members and some activated protein tyrosine kinases including EGFR and insulin/IGF-I receptors. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3, and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 120, 145 |
参考图片Western blot analysis of cell extracts from COS cells overexpressing human FGFR1 and control COS cells, using Phospho-FGF Receptor-1 (Tyr766) (1E5) Rabbit mAb. Before antibody probing, the transferred membrane was untreated (upper) or CIP treated (lower) to confirm the phospho-specificity of the antibody.Western blot法检测以下细胞提取物：高表达人FGFR1的COS细胞和对照COS细胞。使用抗体为：Phospho-FGF Receptor-1 (Tyr766) (1E5) Rabbit mAb。在抗体孵育前，为确定抗体的抗磷酸化蛋白特异性，转了蛋白的膜未经（上）或经过（下）CIP预处理。 |
